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An unbiased genome-wide shRNA screen was performed in A375 human melanoma cells to globally evaluate genetic vulnerabilities to G4-ligands and to identify genes and pathways involved with G4-structures .For this, the pyridine-2,6-bis-quinolino-dicarboxamide derivative, PDS Rodriguez et al., 2012, and bisquinolinium compound, PhenDC3 De Cian et al., 2007b were chosen .
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Recent studies have demonstrated that the human gut is populated by complex, highly individual and stable communities of viruses, the majority of which are bacteriophages. While disease-specific alterations in the gut phageome have been observed in IBD, AIDS and acute malnutrition, the human gut phageome remains poorly characterised. One important obstacle in metagenomic studies of the
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2020-6-7The AP sends a nonce-value to the STA ANonce. The client now has all the attributes to construct the PTK. The STA sends its own nonce-value SNonce to the AP together with a MIC, including authentication, which is really a Message Authentication and Integrity Code MAIC. The AP sends the GTK and a sequence number together with another MIC.
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This work describes the development and characterization of a modular synthetic expression system that provides a broad range of adjustable and predictable expression levels in S. cerevisiae. The system works as a fixed-gain transcription amplifier, where the input signal is transferred via a synthetic transcription factor sTF onto a synthetic promoter, containing a defined core promoter ...
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This joint conference is the one event in 2018 where international researchers, manufacturers, service providers, owners of structures and those active in training, validation and standardization of nondestructive evaluation for civil infrastructure will discuss state-of-the-art as well as innovative NDE technologies, advanced modeling and best practices.
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A 1100 to 150 vv inoculation of 26 L of production culture in LB medium is performed and the culture is grown aerobically with shaking at 37C until the OD 600 is 0.8, then the culture is cooled on ice for 20 min. 4. IPTG is added to a final concentration of 0.25 mM and the culture is grown aerobically at 18C overnight with shaking. 5.
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Tariq et al. disambiguate the allosteric regulation of Hsp104 by several tunable structural contacts, which can be engineered to spawn enhanced therapeutic disaggregases with minimal off-target toxicity. Non-toxic nucleotide-binding domain 1 NBD1 and NBD2 variants emerge that rescue TDP-43, FUS, and -synuclein toxicity connected to neurodegenerative disease.
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Viruses that attack bacteria are known as bacteriophages, or phages for short. Bacteria have developed an antiviral immune system called CRISPR-Cas that works by targeting particular genetic sequences, such as those of an invading phage, for destruction. To counteract this immune system, phages have evolved proteins that can block CRISPR-Cas known as anti-CRISPRs.
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passage library Parallel code generation for hierarchical Bayesian modeling. preprocessor-tools library A framework for extending Haskells syntax via quick-and-dirty preprocessors sugarhaskell programs Library-based syntactic extensibility for Haskell x86-64bit library and test Runtime code generation for x86 64 bit machine code Code ...
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Genome mining of siderophore pathways in 118 closely related Salinispora genomes. Salinispora is a marine actinomycete genus specifically known for its rich biosynthetic capacity to produce ...
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